Fig. 01 -

Title Slide

Fig. 02 -

Disclosures

Fig. 03 -

Topics covered

Fig. 04 -

Topics not covered

Fig. 05 -

Autoimmune Thyroid Disease

Fig. 06 -

Thyroid Autoantibodies

Fig. 07 -

Human TSH Receptor

Fig. 08 -

GPCR

Fig. 09 -

Binding

Fig. 10 -

TSH Signal Transduction

Fig. 11 -

Different types

Fig. 12 -

Stimulating TSHR-A

Fig. 13 -

Blocking TSHR-A

Fig. 14 -

Anti-TSHR Discovery

Fig. 15 -

TSHR Autoantibody Assays

Fig. 16 -

Anti-TSHR Nomenclature

Fig. 17 -

Anti-TSHR (Cont.)

Fig. 18 -

Commercial TRAb assays

Fig. 19 -

TSH-RA Binding Assays

Fig. 20 -

Roche Elecsys

Fig. 21 -

Immulite TSI assay

Fig. 22 -

Siemens Immulite

Fig. 23 -

Be specific

Fig. 24 -

History of Bioassays

Fig. 25 -

Clinically available

Fig. 26 -

Examples of Bioassays

Fig. 27 -

TSAb and TBAb Schematic

Fig. 28 -

Detect only TSI?

Fig. 29 -

Immulite vs Brahms

Fig. 30 -

Another Assay?

Fig. 31 -

Highly variable sensitivity

Fig. 32 -

What happens?

Fig. 33 -

Mix antibodies?

Fig. 34 -

Binding versus Bioassays

Fig. 35 -

Future of Bioassays

Fig. 36 -

Bioassays meet Biosensors

Fig. 37 -

Biosensors

Fig. 38 -

Luminescent Biosensor

Fig. 39 -

Bioassays meet Biosensors

Fig. 40 -

Turbo vs Thyretain

Fig. 41 -

TSI Protocols

Fig. 42 -

Turbo vs Thyretain assays

Fig. 43 -

Summary

Fig. 44 -

Summary (cont’d)

Fig. 45 -

Thank you!